| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; Tslp; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse TSLP, which shares 76.2% amino acid (aa) sequence identity with rat TSLP. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects thymic stromal lymphopoietin (gene Tslp) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 16.2 kDa, and the supplier reports an observed band at about 18 kDa.
Thymic stromal lymphopoietin (TSLP) is an IL-7-like cytokine released mainly by epithelial cells of the skin, airways and gut. It activates dendritic cells, mast cells and other immune cells, promoting type 2 inflammation, and it is a central focus in research on asthma, atopic dermatitis and other allergic diseases.
| Target | Thymic stromal lymphopoietin (gene Tslp; UniProt Q9JIE6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 16.2 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): mouse liver tissue, mouse kidney tissue, mouse testis tissue, rat heart tissue, rat liver tissue, rat kidney tissue, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of mouse TSLP. Sequence identity with the rat ortholog: 76.2%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes thymic stromal lymphopoietin as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
