| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial fission 1 protein; FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11; FIS1; TTC11 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TTC11 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20F49 is a rabbit monoclonal antibody that recognizes mitochondrial fission 1 protein (gene FIS1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 16.9 kDa, and the supplier reports an observed band at about 15 kDa.
Mitochondrial fission 1 protein (FIS1) is a tail-anchored protein of the outer mitochondrial membrane and peroxisomes. It contributes to fragmentation of the mitochondrial network, partly by helping recruit the fission GTPase DRP1, although other adaptors play larger roles in mammals. FIS1 is studied in mitochondrial dynamics, mitophagy and cell stress; it is also conserved in Drosophila.
| Target | Mitochondrial fission 1 protein (gene FIS1; UniProt Q9Y3D6, human) |
|---|---|
| Clone | 20F49 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 16.9 kDa |
| Observed MW | 15 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TTC11.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitochondrial fission 1 protein in the mitochondrion outer membrane and peroxisome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
