| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Nkx-2.1;Homeobox protein NK-2 homolog A;Thyroid nuclear factor 1;Thyroid transcription factor 1;TTF-1;Thyroid-specific enhancer-binding protein;T/EBP;NKX2-1 ;NKX2A, TITF1, TTF1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TTF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27N58 is a rabbit monoclonal antibody that recognizes homeobox protein Nkx-2.1 (gene NKX2-1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 38.6 kDa, and the supplier reports an observed band at about 38–42 kDa.
NKX2-1 (also called TTF-1) is an NK2 homeobox transcription factor that drives thyroid-specific genes such as thyroglobulin, thyroid peroxidase and the TSH receptor. It is also needed for lung development and surfactant protein expression, and it is widely used as a lineage marker for thyroid and lung epithelial cells and their tumors.
| Target | Homeobox protein Nkx-2.1 (gene NKX2-1; UniProt P43699, human) |
|---|---|
| Clone | 27N58 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.6 kDa |
| Observed MW | 38–42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): rat lung cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human TTF1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places homeobox protein Nkx-2.1 in the nucleus. Tissue expression noted by UniProt (human): Thyroid and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
