| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tubby protein homolog; TUB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TUB1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27T22 is a rabbit monoclonal antibody that recognizes tubby protein homolog in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 55.7 kDa, and the supplier reports an observed band at about 55 kDa.
Tubby (TUB) is the founding member of the tubby-like protein family, defined by a C-terminal domain that binds PI(4,5)P2. It helps traffic G protein-coupled receptors to primary cilia and can move from the membrane to the nucleus. Mutation of the mouse gene causes obesity, retinal degeneration and hearing loss.
| Target | Tubby protein homolog (gene TUB; UniProt P50607, human) |
|---|---|
| Clone | 27T22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.7 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TUB1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes tubby protein homolog as secreted and also located in the cytoplasm, nucleus and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
