| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor receptor superfamily member 12A;Fibroblast growth factor-inducible immediate-early response protein 14;FGF-inducible 14;Tweak-receptor;TweakR;CD266;TNFRSF12A;FN14; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human TWEAKR |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TNFRSF12A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone GFF-20; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-TWEAKR Rabbit Monoclonal Antibody catalog # M06261. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A).
- Antibody format: Monoclonal; clone GFF-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TNFRSF12A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. . Reported cellular localization context: Membrane; Single-pass type I membrane protein. Tissue expression notes (as provided): Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
Research relevance and current trends
- Research context keywords from the source record include: Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate TNFRSF12A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TNFRSF12A expression by Western blot in cell or tissue lysates, Detect TNFRSF12A in FFPE tissue sections by immunohistochemistry, Enrich TNFRSF12A by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 70,90 kDa; calculated MW: 13911 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 70,90 kDa
- Cellular localization (provided): Membrane; Single-pass type I membrane protein.
- Tissue details (provided): Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
