| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 12A;Fibroblast growth factor-inducible immediate-early response protein 14;FGF-inducible 14;Tweak-receptor;TweakR;CD266;TNFRSF12A;FN14; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TWEAKR |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GFF-20 is a rabbit monoclonal antibody that recognizes tumor necrosis factor receptor superfamily member 12A (gene TNFRSF12A) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 13.9 kDa; the supplier reports observed bands at 17,20 kDa.
Fn14 (TNFRSF12A) is the receptor for the cytokine TWEAK. It is expressed at low levels in healthy tissue but strongly induced after injury, where it promotes cell proliferation, migration and angiogenesis and can weakly induce apoptosis in some cells. Fn14 is studied in tissue repair, inflammation, muscle regeneration and cancer.
| Target | Tumor necrosis factor receptor superfamily member 12A (gene TNFRSF12A; UniProt Q9NP84, human) |
|---|---|
| Clone | GFF-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 13.9 kDa |
| Observed MW | 17,20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HUVEC cells, human A549 cells, rat PC-12 cells, rat C6 cells, mouse NIH/3T3 cells, mouse 4T1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TWEAKR.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tumor necrosis factor receptor superfamily member 12A in the membrane. Tissue expression noted by UniProt (human): Highly expressed in heart, placenta and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Yang et al. (2024) TWEAK/Fn14 disrupts Th17/Treg balance and aggravates conjunctivitis by inhibiting the Nrf2/HO-1 pathway in allergic conjunctivitis mice. Molecular Medicine. 10.1186/s10020-024-01004-5
Yaping Chen et al. (2025) Isorhamnetin attenuates renal interstitial fibrosis by targeting TWEAK/Fn14-mediated epithelial–mesenchymal transition. Frontiers in Immunology. 10.3389/fimmu.2025.1649327
Haiyan He et al. (2023) Ginkgolide A downregulates transient receptor potential (melastatin) 2 to protect cisplatin-induced acute kidney injury in rats through the TWEAK/Fn14 pathway: Ginkgolide A improve acute renal injury. Human & Experimental Toxicology. 10.1177/09603271231200868
