| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thioredoxin domain-containing protein 5; Endoplasmic reticulum resident protein 46; ER protein 46; ERp46; Thioredoxin-like protein p46; TXNDC5; TLP46 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TXNDC5 recombinant protein (Position: M109-R403). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes thioredoxin domain-containing protein 5 (gene TXNDC5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 47.6 kDa, and the supplier reports an observed band at about 50–55 kDa.
TXNDC5 (also called ERp46) is a protein disulfide isomerase family member located in the endoplasmic reticulum. It has three thioredoxin-like domains and helps form correct disulfide bonds in newly made secretory and membrane proteins, and it is studied in ER stress, fibrosis and cancer.
| Target | Thioredoxin domain-containing protein 5 (gene TXNDC5; UniProt Q8NBS9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 47.6 kDa |
| Observed MW | 50–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human A549 cells, human HepG2 cells, rat stomach tissue, mouse ovary tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human larynx squamous cells carcinoma tissue, human prostate adenocarcinoma tissue, human rectum adenocarcinoma tissue, human renal cancer tissue, human spleen tissue, human thyroid papillary carcinoma tissue, rat colon tissue, mouse spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human colon cancer tissue.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human TXNDC5 fragment (Met109–Arg403), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places thioredoxin domain-containing protein 5 in the endoplasmic reticulum lumen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
