| Field | Specification |
|---|---|
| Target | |
| Alternative names | Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit; U2AF2; U2AF65 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human U2AF65 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21U21 is a rabbit monoclonal antibody that recognizes splicing factor U2AF 65 kDa subunit (gene U2AF2) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 53.5 kDa, and the supplier reports an observed band at about 60 kDa.
U2AF2 (U2AF65) is the large subunit of U2 auxiliary factor, which binds the polypyrimidine tract near the 3′ splice site of introns. With U2AF1 and SF1 it helps recognize the 3′ splice site and recruit the U2 snRNP during early spliceosome assembly, and it also influences alternative splicing and mRNA export.
| Target | Splicing factor U2AF 65 kDa subunit (gene U2AF2; UniProt P26368, human) |
|---|---|
| Clone | 21U21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.5 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human U2AF65.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places splicing factor U2AF 65 kDa subunit in the nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
