| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kinesin-like protein KIF23;Kinesin-like protein 5;Mitotic kinesin-like protein 1;KIF23;KNSL5, MKLP1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human UAP56 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-DDX39B antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23D06; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-UAP56 Rabbit Monoclonal Antibody catalog # M03490-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: DDX39B (Kinesin-like protein KIF23).
- Antibody format: Monoclonal; clone 23D06; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
DDX39B (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-ed motor enzyme that moves antiparallel microtubules in vitro. . Reported cellular localization context: Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis. Tissue expression notes (as provided): Expressed in the normal brain and also in malignant gliomas (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: Cell Type Markers,Immunology.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate DDX39B antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DDX39B expression by Western blot in cell or tissue lysates, Detect DDX39B in FFPE tissue sections by immunohistochemistry, Localize DDX39B by immunofluorescence/immunocytochemistry in cultured cells, Quantify DDX39B-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 45 kDa; calculated MW: 110059 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 45 kDa
- Cellular localization (provided): Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to the interzone of mitotic spindles. Detected at the midbody during later stages of mitotic cytokinesis.
- Tissue details (provided): Expressed in the normal brain and also in malignant gliomas (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
