| Field | Specification |
|---|---|
| Target | |
| Alternative names | Polyubiquitin-B;Ubiquitin;UBB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Ubiquitin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IBI-21 is a rabbit monoclonal antibody that recognizes polyubiquitin-B (gene UBB) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 25.8 kDa, and the supplier reports an observed band at about 26 kDa.
UBB is one of two human polyubiquitin genes, encoding three ubiquitin units in tandem that are cleaved into single ubiquitin molecules. Ubiquitin is attached to target proteins as single units or chains, and different chain types signal proteasomal degradation, DNA repair, endocytosis or activation of signaling pathways such as NF-κB.
| Target | Polyubiquitin-B (gene UBB; UniProt P0CG47, human) |
|---|---|
| Clone | IBI-21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 25.8 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): Jurkat cells.
Immunogen
Synthetic peptide based on the sequence of human ubiquitin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places polyubiquitin-B in the [Ubiquitin]: Cytoplasm, nucleus and mitochondrion outer membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
