Anti-UCHL5IP Rabbit Monoclonal Antibody

SKU:BHA21010537
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Boster Bio
Boster Bio
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Overview
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Anti-HAUS7 antibody (Rabbit monoclonal, clone 30H47, IgG). Validated for Western blot, immunohistochemistry and immunoprecipitation. Reactivity: Human. Recognizes HAUS augmin-like complex subunit 7. Observed band in Western blot: about 37 kDa.
Target HAUS7
clone number 30H47
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, IHC, IP
Options selector
Catalog no. Size Conjugation
M12338-2 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB,IHC,IP
      • Application details: WB 1:500-2000
        IHC 1:50-200
        IP 1:50
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HAUS7
Alternative names HAUS augmin-like complex subunit 7; 26S proteasome-associated UCH37-interacting protein 1; UCHL5-interacting protein; X-linked protein STS1769; HAUS7; UCHL5IP; UIP1
UniProt # Q99871
Host Rabbit
Clone 30H47
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human UCHL5IP
Molecular weight 40.8 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus . Cytoplasm .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M12338-2
Main SKU BHA21010537
Rabbit Monoclonal

Product Overview

Clone 30H47 is a rabbit monoclonal antibody that recognizes HAUS augmin-like complex subunit 7 (gene HAUS7) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 40.8 kDa, and the supplier reports an observed band at about 37 kDa.

HAUS7 is a subunit of the HAUS (augmin) complex, which promotes microtubule nucleation from existing spindle microtubules during mitosis. The complex helps build a robust spindle, maintain centrosome integrity and complete cytokinesis. HAUS7 is studied in cell division and microtubule organization.

Target HAUS augmin-like complex subunit 7 (gene HAUS7; UniProt Q99871, human)
Clone 30H47
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 40.8 kDa
Observed MW 37 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunoprecipitation 1:50

Samples with a confirmed band (WB): Jurkat cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human UCHL5IP.

Reactivity Notes

The supplier lists reactivity with human. UniProt places HAUS augmin-like complex subunit 7 in the cytoplasm. Tissue expression noted by UniProt (human): Detected in spleen, thymus, testis, ovary, small intestine and colon, with highest levels of expression in testis and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 40.8 kDa; observed band at about 37 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human UCHL5IP.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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