| Field | Specification |
|---|---|
| Target | |
| Alternative names | Putative mitochondrial transporter UCP3; Solute carrier family 25 member 9; Uncoupling protein-3; UCP 3; UCP3; SLC25A9 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human UCP3, which shares 73.1% and 76.9% amino acid (aa) sequence identity with mouse and rat UCP3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes putative mitochondrial transporter UCP3 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 34.2 kDa, and the supplier reports an observed band at about 30 kDa.
UCP3 is a mitochondrial inner-membrane carrier found mainly in skeletal muscle and brown fat. It was first proposed to uncouple respiration by leaking protons, but this role is debated and its exact transport function remains unclear. UCP3 is studied in fatty acid metabolism, energy balance and muscle physiology.
| Target | Putative mitochondrial transporter UCP3 (gene UCP3; UniProt P55916, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 34.2 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, rat L6 cells, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human UCP3. Sequence identity with the mouse and rat orthologs: 73.1% and 76.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places putative mitochondrial transporter UCP3 in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Only in skeletal muscle and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
