| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase UHRF1; Inverted CCAAT box-binding protein of 90 kDa; Nuclear zinc finger protein Np95; HuNp95; Nuclear protein 95; hNp95; RING finger protein 106; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; hUHRF1; UHRF1; ICBP90; NP95; RNF106 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human UHRF1 recombinant protein (Position: K592-R793). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets E3 ubiquitin-protein ligase UHRF1 in human and mouse samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 89.8 kDa, and the supplier reports an observed band at about 100 kDa.
UHRF1 is an epigenetic regulator and E3 ubiquitin ligase that is essential for maintaining DNA methylation through cell division. It binds hemimethylated DNA at replication forks and specific histone H3 marks, then ubiquitinates histone H3 to recruit DNMT1, which copies methylation onto the new strand. UHRF1 is studied in epigenetics, development and cancer.
| Target | E3 ubiquitin-protein ligase UHRF1 (gene UHRF1; UniProt Q96T88, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 89.8 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human A549 cells, mouse SP2/0 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
Recombinant human UHRF1 fragment (Lys592–Arg793), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places E3 ubiquitin-protein ligase UHRF1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in thymus, bone marrow, testis, lung and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
