| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase ULK1;2.7.11.1;Autophagy-related protein 1 homolog;ATG1;hATG1;Unc-51-like kinase 1;ULK1;KIAA0722; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ULK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABHH-21 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase ULK1 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 112.6 kDa, and the supplier reports an observed band at about 130 kDa.
ULK1 is a serine/threonine kinase that starts autophagy when cells face nutrient shortage. Inhibited by mTORC1 and activated by AMPK, it forms a complex with ATG13 and FIP200 and acts upstream of the PI3-kinase VPS34 to launch autophagosome formation. ULK1 is a central target in autophagy, metabolism and cancer research.
| Target | Serine/threonine-protein kinase ULK1 (gene ULK1; UniProt O75385, human) |
|---|---|
| Clone | ABHH-21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 112.6 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human 293T cells, human A549 cells, human U-87MG cells, human HeLa cells, rat liver tissue, rat PC-12 cells, mouse liver tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue, human spleen tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): 293 cells.
Immunogen
Synthetic peptide based on the sequence of human ULK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase ULK1 in the cytoplasm and preautophagosomal structure. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhou Cefan et al. (2020) LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. Molecular Cancer. 10.1186/s12943-020-01237-y
Cefan Zhou et al. (2019) STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. Autophagy. 10.1080/15548627.2019.1687212
Junjun Guo et al. (2025) TIGAR deficiency exacerbated preeclampsia by impairing autophagy and accelerating trophoblasts apoptosis upon excessive oxidative stress via the NRF2-ARE pathway. Life Sciences. 10.1016/j.lfs.2025.124062
Jing Shi et al. (2025) Fucoidan Oligosaccharide Supplementation Relieved Kidney Injury and Modulated Intestinal Homeostasis in D-Galactose-Exposed Rats. Nutrients. 10.3390/nu17020325
