| Field | Specification |
|---|---|
| Target | |
| Alternative names | Urokinase plasminogen activator surface receptor;U-PAR;uPAR;Monocyte activation antigen Mo3;CD87;PLAUR;MO3, UPAR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PLAUR recombinant protein (Position: E230-S286). Human PLAUR shares 63.2% amino acid (aa) sequence identity with both mouse and rat PLAUR. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects urokinase plasminogen activator surface receptor (gene PLAUR) in human samples and is validated for Western blot. The predicted molecular weight is 37.0 kDa; the supplier reports observed bands at 39 kDa, 55 kDa.
The urokinase receptor (uPAR, CD87) is a GPI-anchored cell surface receptor that binds uPA and focuses plasminogen activation at the cell surface. It also binds vitronectin and signals through integrins, so it coordinates proteolysis, adhesion and migration, and it is studied in inflammation, cancer invasion and kidney disease.
| Target | Urokinase plasminogen activator surface receptor (gene PLAUR; UniProt Q03405, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.0 kDa |
| Observed MW | 39 kDa, 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human PLAUR fragment (Glu230–Ser286), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 63.2%.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed in neurons of the rolandic area of the brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
