| Field | Specification |
|---|---|
| Target | |
| Alternative names | Urokinase plasminogen activator surface receptor; U-PAR; Monocyte activation antigen Mo3; uPAR; CD87; Plaur; MO3; UPAR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human uPA Receptor/PLAUR. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes urokinase plasminogen activator surface receptor (gene PLAUR) in human, mouse and rat samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 37.0 kDa, and the supplier reports an observed band at about 39 kDa.
The urokinase receptor (uPAR, CD87) is a GPI-anchored cell surface receptor that binds uPA and focuses plasminogen activation at the cell surface. It also binds vitronectin and signals through integrins, so it coordinates proteolysis, adhesion and migration, and it is studied in inflammation, cancer invasion and kidney disease.
| Target | Urokinase plasminogen activator surface receptor (gene PLAUR; UniProt Q03405, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.0 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells106 cells |
Samples with a confirmed band (WB): human placenta tissue, human U2OS cells, human A431 cells, human HeLa cells, human A549 cells, rat testis tissue, mouse small intestine tissue, mouse kidney tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Synthetic peptide from the C-terminal region of human uPA Receptor/PLAUR.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed in neurons of the rolandic area of the brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
