| Field | Specification |
|---|---|
| Target | |
| Alternative names | Regulator of nonsense transcripts 3B;Nonsense mRNA reducing factor 3B;Up-frameshift suppressor 3 homolog B;hUpf3B;Up-frameshift suppressor 3 homolog on chromosome X;hUpf3p-X;UPF3B;RENT3B, UPF3X; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human UPF3B /RENT3B. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody detects regulator of nonsense transcripts 3B (gene UPF3B) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 57.8 kDa, and the supplier reports an observed band at about 58 kDa.
UPF3B is a nonsense-mediated mRNA decay (NMD) factor that links the exon junction complex on spliced mRNA to UPF2 and UPF1. This connection helps mark transcripts with premature stop codons for degradation. UPF3B is X-linked, and its mutations are associated with intellectual disability, so it is studied in mRNA surveillance and neurodevelopment.
| Target | Regulator of nonsense transcripts 3B (gene UPF3B; UniProt Q9BZI7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 57.8 kDa |
| Observed MW | 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): human HeLa cells, human 22RV1 cells, human U87 cells, human Jurkat cells, rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse testis tissue, rat testis tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human UPF3B /RENT3B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places regulator of nonsense transcripts 3B in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huang Lulu et al. (2018) Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biology. 10.1186/s13059-017-1386-9
Ghiasi Seyed M. et al. (2024) Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells. Frontiers in Endocrinology. 10.3389/fendo.2024.1359147
Wentao Shi et al. (2019) Clobetasol propionate enhances neural stem cell and oligodendrocyte differentiation. Experimental and Therapeutic Medicine. 10.3892/etm.2019.7692
