| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome b-c1 complex subunit 2, mitochondrial; Complex III subunit 2; Core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2; UQCRC2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human UQCRC2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADBG-21 is a rabbit monoclonal antibody that recognizes cytochrome b-c1 complex subunit 2, mitochondrial (gene UQCRC2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 48.4 kDa, and the supplier reports an observed band at about 48 kDa.
UQCRC2 is a core subunit of mitochondrial complex III (the cytochrome b-c1 complex) in the electron transport chain. Complex III transfers electrons from ubiquinol to cytochrome c while pumping protons, contributing to ATP production. UQCRC2 is a common marker in studies of oxidative phosphorylation and mitochondrial function.
| Target | Cytochrome b-c1 complex subunit 2, mitochondrial (gene UQCRC2; UniProt P22695, human) |
|---|---|
| Clone | ADBG-21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.4 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human 293T cells, human HepG2 cells, human K562 cells, human Hacat cells, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human UQCRC2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome b-c1 complex subunit 2, mitochondrial in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
