Anti-USO1 Rabbit Monoclonal Antibody

Boster Bio Anti-USO1 Rabbit Monoclonal Antibody catalog # M05911. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Protein: T-cell surface glycoprotein CD3 zeta chain | Gene: Golgin subfamily A member 2 | Target: USO1 | Function: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin- alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401). . | Research areas: Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction,Cancer,Cell Biology,Energy Transfer Pathways,Cancer Metabolism,Metabolic Signaling Pathway,Oxidative Stress,Redox Metabolism,Mitochondrial Metabolism,Mitochondrial,Mitochondrial Markers,Integration Of Energy,Integration Of Energy Metabolism,Oxidative Phosphorylation

Host: Rabbit | Clonality/Clone: Monoclonal / Clone: 29U63 | Isotype: IgG | Reactivity: Human,Mouse,Rat | Immunogen: A synthesized peptide derived from human USO1 | Concentration: 0.5mg/ml | Purification: Affinity-chromatography

Applications: WB, IHC, IF, ICC

Observed MW: 108 kDa | Calculated MW: 113086 MW

Cellular localization: Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, spindle pole . Peripheral membrane protein associated with cis-Golgi stacks (By similarity). Associates with the mitotic spindle during mitosis (PubMed:26165940). .

Tissue details: Expressed in all tissues examined.

Status: Active

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