| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B-lymphocyte antigen CD20;B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;CD20;MS4A1;CD20; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human USP10 Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-USP10 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADCB-21; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-USP10 Monoclonal Antibody catalog # M03786. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: USP10 (B-lymphocyte antigen CD20).
- Antibody format: Monoclonal; clone ADCB-21; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
USP10 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): This protein may be involved in the regulation of B-cell activation and proliferation. Reported cellular localization context: Cell membrane ; Multi-pass membrane protein . Cell membrane ; Lipid-anchor . Tissue expression notes (as provided): Expressed on B-cells.
Research relevance and current trends
- Research context keywords from the source record include: Cell Biology,Deubiquitination,Epigenetics and Nuclear Signaling,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Ubiquitin & Ubiquitin Like Modifiers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate USP10 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect USP10 expression by Western blot in cell or tissue lysates, Detect USP10 in FFPE tissue sections by immunohistochemistry, Localize USP10 by immunofluorescence/immunocytochemistry in cultured cells, Enrich USP10 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 25 kDa; calculated MW: 33077 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 25 kDa
- Cellular localization (provided): Cell membrane ; Multi-pass membrane protein . Cell membrane ; Lipid-anchor .
- Tissue details (provided): Expressed on B-cells.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
