| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin carboxyl-terminal hydrolase 22; Deubiquitinating enzyme 22; Ubiquitin thioesterase 22; Ubiquitin-specific-processing protease 22; USP22; KIAA1063; USP3L |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human USP22, which shares 89.5% amino acid (aa) sequence identity with mouse USP22. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ubiquitin carboxyl-terminal hydrolase 22 (gene USP22) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 60.0 kDa, and the supplier reports an observed band at about 68 kDa.
USP22 is the deubiquitinating enzyme of the SAGA transcription coactivator complex, where it removes ubiquitin from histones H2A and H2B. It is recruited to target promoters by activators such as MYC and supports their transcription. USP22 is studied in gene regulation, cell-cycle progression and cancer.
| Target | Ubiquitin carboxyl-terminal hydrolase 22 (gene USP22; UniProt Q9UPT9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.0 kDa |
| Observed MW | 68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human A549 cells, human HepG2 cells, human HeLa cells, human Caco-2 cells, human MOLT-4 cells, human SH-SY5Y cells, human U251 cells, rat liver tissue, rat heart tissue, rat kidney tissue, rat RH35 cells, mouse liver tissue, mouse heart tissue, mouse kidney tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human USP22. Sequence identity with the mouse ortholog: 89.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin carboxyl-terminal hydrolase 22 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
