Description
Anti-V5 Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with Sepharose 4B gel. This product has a high V5 label fusion protein loading capacity (at least 0.5 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with V5 labels.
Features
- Specific—highly specific monoclonal antibody
- High yield and high purity
- Versatile—It can be used for protein purification or COIP
- Convenient—reagents and peptides to elute are available separately
Applications
- Immunoprecipitation (IP)
- Purification of fusion proteins with V5 labels.
Specifications
| Clone | 10B5 |
| Isotype | Mouse IgG1 |
| Purity | Protein A |
| Antibody Concentration | 7.5 g antibody/L Gel |
| Protein binding capacity | ≥0.5 mg protein/mL Gel |
| Storage buffer | 10 mM Na3PO4, 150mM NaCl, 50% glycerin, pH7.4, contains 0.02% (w/v) sodium azide |
Components
| Components No. | Name | 20588ES03 | 20588ES08 | 20588ES25 | 20588ES60 |
| 20588 | Anti-V5 Affinity Gel | 1 mL | 5 mL | 25 mL | 100 mL |
Storage
The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!
The V5 tag is a 14-amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of paramyxovirus SV5. The antibody is clone 10B5, isotype Mouse IgG1, purified by Protein A. Antibody concentration: 7.5 g antibody/L gel. Protein binding capacity: ≥0.5 mg V5-tagged protein per mL gel.
V5 is commonly used for cell-based expression studies, pull-downs, and protein–protein interaction studies. Because V5 is derived from a viral protein, it has no mammalian counterpart, minimizing background from endogenous proteins in mammalian cell lysates. It is used in both prokaryotic and eukaryotic expression systems.
The Anti-V5 gel has the lowest binding capacity in this series at ≥0.5 mg/mL, compared to HA (≥1.5 mg/mL), His (≥1.1 mg/mL), Flag (≥1.1 mg/mL), and Myc (≥0.9 mg/mL). Plan your experiment accordingly — use a proportionally larger gel volume if your target protein is highly expressed.
Yes — the 100 mL format (20588ES60) is available for preparative-scale purification of V5-tagged proteins. The gel can be packed into chromatography columns or used in batch mode. Ensure proper column equilibration and optimized wash conditions to maintain purity.
Unopened: −25°C to −15°C for 1 year. After opening: 2–8°C. Storage buffer: 10 mM Na₃PO₄, 150 mM NaCl, 50% glycerol, pH 7.4, 0.02% sodium azide. Do not freeze without glycerol-containing buffer.
The following customization and add-on services may be available for this product type through the supplier. For inquiries and pricing, contact support@biohippo.com.
Customization Options
- Bulk Quantities: Larger volumes and custom pack sizes beyond the standard catalog may be available for high-throughput core facilities and industrial customers. Contact us to inquire about bulk pricing.
- Custom Antibody Coupling (Magnetic Beads): Custom magnetic beads conjugated to your specific antibody or protein ligand may be available through Yeasen's OEM and custom services program. These services leverage the same bead platform used for catalog products.
- OEM Manufacturing: Custom bead formulations for diagnostic device development, including magnetic beads for nucleic acid isolation, IP-grade beads, and affinity capture beads, may be available at manufacturing scale.
To inquire about custom options or bulk pricing, contact support@biohippo.com.
