| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vesicle-associated membrane protein-associated protein A; VAMP-A; VAMP-associated protein A; VAP-A; 33 kDa VAMP-associated protein; VAP-33; VAPA; VAP33 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human VAPA, which shares 94.1% amino acid (aa) sequence identity with mouse and rat VAPA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects vesicle-associated membrane protein-associated protein A (gene VAPA) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 27.9 kDa, and the supplier reports an observed band at about 33 kDa.
VAPA is an endoplasmic reticulum membrane protein that forms contact sites between the ER and other organelles. It binds proteins carrying FFAT motifs, such as OSBP and STARD3, which transfer lipids like cholesterol between membranes. VAPA is studied in membrane contact sites, lipid transport and vesicle trafficking.
| Target | Vesicle-associated membrane protein-associated protein A (gene VAPA; UniProt Q9P0L0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.9 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human 293T cells, human HepG2 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human cervical cancer tissue, human colorectal adenocarcinoma tissue, human lung adenocarcinoma tissue, human placenta tissue, mouse colon tissue, rat colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with confirmed staining (ICC/IF): human colorectal adenocarcinoma tissue, rat colon tissue.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Synthetic peptide from the N-terminal region of human VAPA. Sequence identity with the mouse and rat orthologs: 94.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places vesicle-associated membrane protein-associated protein A in the endoplasmic reticulum membrane, cell membrane, cell junction and nuclear membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
