| Field | Specification |
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| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q). |
| Purification | |
| Concentration | |
| Formulation | |
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Product Overview
This polyclonal antibody, raised in Rabbit, recognizes VASP. VASP (phospho-Ser238) antibody detects endogenous levels of VASP only when phosphorylated at serine 238. It is validated for Western Blot, Immunohistochemistry.
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involve
Background
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
Validated Applications
| Applications | Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:500~1:1000 IHC: 1:50~1:100 |
WB: 1:500~1:1000 IHC: 1:50~1:100
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q).
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q). |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
| Specificity | VASP (phospho-Ser238) antibody detects endogenous levels of VASP only when phosphorylated at serine 238. |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Storage Buffer | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Shipping | Ice Pack |
Reactivity Notes
VASP (phospho-Ser238) antibody detects endogenous levels of VASP only when phosphorylated at serine 238.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. Contains sodium azide where stated; handle with standard laboratory precautions.
Related Products
Explore related antibodies and detection reagents for VASP research from Applied Biological Materials (abm) Inc., or contact us for alternative conjugates and formats.
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