| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vasodilator-stimulated phosphoprotein;VASP;VASP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VASP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BHO-22 is a rabbit monoclonal antibody that recognizes vasodilator-stimulated phosphoprotein (gene VASP) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 39.8 kDa, and the supplier reports an observed band at about 40 kDa.
VASP is a member of the Ena/VASP family of actin regulators. It promotes actin filament elongation by protecting growing barbed ends from capping proteins, and it acts at focal adhesions, lamellipodia and filopodia. VASP phosphorylation by cAMP- and cGMP-dependent kinases is a common readout in platelet and vascular signaling studies.
| Target | Vasodilator-stimulated phosphoprotein (gene VASP; UniProt P50552, human) |
|---|---|
| Clone | BHO-22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.8 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human VASP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places vasodilator-stimulated phosphoprotein in the cytoplasm, cell junction and cell projection. Tissue expression noted by UniProt (human): Highly expressed in platelets.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
