| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; VCAM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human VCAM1 recombinant protein (Position: F25-L270). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets vascular cell adhesion protein 1 (gene VCAM1) in human samples and is validated for ELISA and IHC. The predicted molecular weight is 81.3 kDa.
VCAM-1 (CD106) is an immunoglobulin-superfamily adhesion molecule expressed on activated endothelial cells. Induced by inflammatory cytokines, it binds the integrin VLA-4 (α4β1) on leukocytes and supports their firm adhesion and migration into tissues. VCAM-1 is a common marker of endothelial activation in inflammation and atherosclerosis research.
| Target | Vascular cell adhesion protein 1 (gene VCAM1; UniProt P19320, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 81.3 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with confirmed staining (IHC): human appendix tissue, human tonsil tissue, human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human VCAM1 fragment (Phe25–Leu270), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Wu-Yang Huang et al. (2014) Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells. Molecules. 10.3390/molecules190812827
Wu-Yang Huang et al. (2017) Quercetin, Hyperin, and Chlorogenic Acid Improve Endothelial Function by Antioxidant, Antiinflammatory, and ACE Inhibitory Effects. Journal of Food Science. 10.1111/1750-3841.13706
Wu-Yang Huang et al. (2018) Malvidin and its Glycosides from Vaccinium ashei Improve Endothelial Function by Anti-inflammatory and Angiotensin I-Converting Enzyme Inhibitory Effects. Natural Product Communications. 10.1177/1934578X1801300115
