| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cadherin-5;Vascular endothelial cadherin;VE-cadherin;CD144;Cdh5; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from mouse VE Cadherin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IIC-3 is a rabbit monoclonal antibody that recognizes cadherin-5 (gene Cdh5) in mouse samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 87.9 kDa, and the supplier reports an observed band at about 88 kDa.
VE-cadherin (cadherin-5, CDH5) is the main cadherin of vascular endothelial cells, where it builds the adherens junctions that hold neighboring endothelial cells together. By controlling junction stability it governs vessel integrity and permeability, making it a standard endothelial marker in studies of angiogenesis, vascular leakage and inflammation.
| Target | Cadherin-5 (gene Cdh5; UniProt P55284, mouse) |
|---|---|
| Clone | IIC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 87.9 kDa |
| Observed MW | 88 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): mouse lung.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of mouse VE Cadherin.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places cadherin-5 in the cell junction, cell membrane and cytoplasm. Tissue expression noted by UniProt (mouse): Expressed in postnatal endothelial cells of the retinal vascular plexus (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lai Wei et al. (2022) Chitosan/Alginate Hydrogel Dressing Loaded FGF/VE-Cadherin to Accelerate Full-Thickness Skin Regeneration and More Normal Skin Repairs. International Journal of Molecular Sciences. 10.3390/ijms23031249
Jiang Jingtao et al. (2021) Positive Effect of Andrographolide Induced Autophagy on Random-Pattern Skin Flaps Survival. Frontiers in Pharmacology. 10.3389/fphar.2021.653035
Jinti Lin et al. (2019) Protective effects of resveratrol on random-pattern skin flap survival: an experimental study. American Journal of Translational Research. PMID: 30787995
Yuedong Fu et al. (2025) Fisetin promotes ischemic flap survival by enhancing autophagy via inhibition of the PI3K/Akt/mTOR signaling pathway. Archives of Biochemistry and Biophysics. 10.1016/j.abb.2025.110716
Lingxian Yi et al. (2022) Overexpression of interleukin-10 in engineered macrophages protects endothelial cells against LPS-induced injury in vitro. FEBS Open Bio. 10.1002/2211-5463.13365
