| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor; FLT1; FLT, FRT, VEGFR1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
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| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human VEGF Receptor 1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
This rabbit polyclonal antibody targets vascular endothelial growth factor receptor 1 (gene FLT1) in human, mouse and rat samples and is validated for ELISA, flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 150.8 kDa, and the supplier reports an observed band at about 125 kDa.
Vascular endothelial growth factor receptor 1 (VEGFR-1, FLT1) is a receptor tyrosine kinase that binds VEGF-A, VEGF-B and placental growth factor. Its kinase signaling is weak, and a soluble form acts largely as a decoy that limits VEGF availability. FLT1 is studied in angiogenesis, macrophage recruitment, preeclampsia and vascular development.
| Target | Vascular endothelial growth factor receptor 1 (gene FLT1; UniProt P17948, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 150.8 kDa |
| Observed MW | 125 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human placenta tissue, human A549 cells, human COLO-320 cells, human A431 cells, human SK-OV-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human VEGF Receptor 1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chaojun Tang et al. (2010) The impact of vascular endothelial growth factor-transfected human endothelial cells on endothelialization and restenosis of stainless steel stents. Journal of Vascular Surgery. 10.1016/j.jvs.2010.08.020
