| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; CD309; Kdr; Flk-1; Flk1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse VEGF Receptor 2 recombinant protein (Position: A20-L244). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes vascular endothelial growth factor receptor 2 (gene Kdr) in mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 152.5 kDa, and the supplier reports an observed band at about 150 kDa.
VEGFR2 (KDR, also called FLK1) is a receptor tyrosine kinase on endothelial cells that binds VEGF-A as well as processed VEGF-C and VEGF-D. Ligand binding triggers receptor dimerization and autophosphorylation, activating PLCγ, PI3K/AKT and MAPK signaling that drives endothelial proliferation, migration, survival and vascular permeability. VEGFR2 is the main mediator of VEGF-driven angiogenesis and a major target of anti-angiogenic cancer drugs.
| Target | Vascular endothelial growth factor receptor 2 (gene Kdr; UniProt P35918, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 152.5 kDa |
| Observed MW | 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): Marker, mouse heart tissue, mouse thymus tissue, mouse spleen tissue, mouse kidney tissuemouse brain tissue, mouse lung tissuemouse HEPA1–6 cells, mouse NIH3T3 whole cellThe membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901–3) at 10 µg/mL overnight at 4 °C, then washed with TBS-01%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 15 hour at RTThe signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 50 µg lysate per lane, primary antibody at 1.0 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): NIH3T3 cells.
Samples with a confirmed signal (flow cytometry): MFC cells, LLC cells.
Immunogen
Recombinant mouse VEGF Receptor 2 fragment (Ala20–Leu244), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places vascular endothelial growth factor receptor 2 in the cell junction, endoplasmic reticulum and cell membrane. Tissue expression noted by UniProt (mouse): Expressed in endothelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
He Wenjun et al. (2022) Molecular Mechanism of Naringenin Against High-Glucose-Induced Vascular Smooth Muscle Cells Proliferation and Migration Based on Network Pharmacology and Transcriptomic Analyses. Frontiers in Pharmacology. 10.3389/fphar.2022.862709
Tingting Gao et al. (2024) The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity Through the cAMP/Rac1 Pathway. International Journal of Molecular Sciences. 10.3390/ijms252011110
Lujie Ye et al. (2024) Dysregulated VEGF/VEGFR-2 Signaling and Plexogenic Lesions in the Embryonic Lungs of Chickens Predisposed to Pulmonary Arterial Hypertension. International Journal of Molecular Sciences. 10.3390/ijms25084489
Gao Ranran et al. (2025) Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway. Journal of Ovarian Research. 10.1186/s13048-025-01679-2
Zishuo Li et al. (2024) EPAC inhibitor suppresses angiogenesis and tumor growth of triple-negative breast cancer. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2024.167114
Liu Siqi et al. (2026) 5-HMF inhibits glucocorticoid-induced osteoporosis through the VEGFR2/PI3K/AKT pathway. Scientific Reports. 10.1038/s41598-026-44463-y
Yanfang Gao et al. (2016) Arsenic-induced anti-angiogenesis via miR-425-5p-regulated CCM3. Toxicology Letters. 10.1016/j.toxlet.2016.04.023
Ning Kun Zhang et al. (2016) Activation of Endogenous Cardiac Stem Cells by Apelin-13 in Infarcted Rat Heart. Cell Transplantation. 10.3727/096368916X691123
Erlin Zhang et al. (2015) A ferulic acid (FA)-eluting system for biodegradable magnesium stent: Cells response of HUVECs. Journal of Biomedical Materials Research Part a. 10.1002/jbm.a.35410
Quan-Li Li et al. (2010) Oriented immobilization of anti-CD34 antibody on titanium surface for self-endothelialization induction. Journal of Biomedical Materials Research Part a. 10.1002/jbm.a.32812
