| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD309;KDR;FLK1, VEGFR2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VEGF Receptor 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CIH-11 is a rabbit monoclonal antibody that recognizes vascular endothelial growth factor receptor 2 (gene KDR) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 151.5 kDa, and the supplier reports an observed band at about 180–250 kDa.
VEGFR2 (KDR, also called FLK1) is a receptor tyrosine kinase on endothelial cells that binds VEGF-A as well as processed VEGF-C and VEGF-D. Ligand binding triggers receptor dimerization and autophosphorylation, activating PLCγ, PI3K/AKT and MAPK signaling that drives endothelial proliferation, migration, survival and vascular permeability. VEGFR2 is the main mediator of VEGF-driven angiogenesis and a major target of anti-angiogenic cancer drugs.
| Target | Vascular endothelial growth factor receptor 2 (gene KDR; UniProt P35968, human) |
|---|---|
| Clone | CIH-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 151.5 kDa |
| Observed MW | 180–250 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human VEGF Receptor 2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places vascular endothelial growth factor receptor 2 in the cell junction, endoplasmic reticulum and cell membrane. Tissue expression noted by UniProt (human): Detected in cornea (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zheng Zeng et al. (2023) Enterolactone and trabectedin suppress epithelial ovarian cancer synergistically via upregulating THBS1. Phytotherapy Research. 10.1002/ptr.7942
Jinjin Sun et al. (2019) Downregulation of miR-21 inhibits the malignant phenotype of pancreatic cancer cells by targeting VHL. OncoTargets and Therapy. 10.2147/OTT.S211535
