| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;VEGFA;VEGF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VEGF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADB-22 is a rabbit monoclonal antibody that recognizes vascular endothelial growth factor A, long form (gene VEGFA) in human and mouse samples and is validated for IHC, ICC/IF and flow cytometry. The predicted molecular weight is 27.0 kDa, and the supplier reports an observed band at about 28 kDa.
VEGF-A is the main vascular endothelial growth factor that drives angiogenesis. Produced in response to hypoxia, it binds VEGFR1 and VEGFR2 on endothelial cells to promote their proliferation, migration and survival and to increase vascular permeability. VEGF-A is widely studied in vascular development, wound healing, eye disease and tumor angiogenesis.
| Target | Vascular endothelial growth factor A, long form (gene VEGFA; UniProt P15692, human) |
|---|---|
| Clone | ADB-22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.0 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): human kindey tissue, human breast cancer tissue, human colon cancer tissue, human endometrial cancer tissue, mouse kidney tissue, mouse pancreas tissue, human ovarian cancer tissue, human prostatic cancer tissue, rat thyroid tissue, human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human breast cancer tissue, human endometrial cancer tissue, human ovarian cancer tissue, human pancreatic cancer tissue, human prostatic cancer tissue, human thyroid cancer tissue.
Immunogen
Synthetic peptide based on the sequence of human VEGF.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places vascular endothelial growth factor A, long form in the [N-VEGF]: Cytoplasm and nucleus. Tissue expression noted by UniProt (human): Higher expression in pituitary tumors than the pituitary gland...
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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