| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor D; VEGF-D; c-Fos-induced growth factor; FIGF; VEGFD |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VEGFD |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25V50 is a rabbit monoclonal antibody that recognizes vascular endothelial growth factor D (gene VEGFD) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 40.4 kDa, and the supplier reports an observed band at about 38 kDa.
VEGF-D is a growth factor of the VEGF family that binds and activates VEGFR-2 and VEGFR-3. It promotes the growth of blood and lymphatic vessels, with a particular role in lymphangiogenesis, and it is studied in tumor lymphatic spread and in vascular development.
| Target | Vascular endothelial growth factor D (gene VEGFD; UniProt O43915, human) |
|---|---|
| Clone | 25V50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.4 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Caco-2 cells, human HepG2 cells, human A549 cells, human HeLa cells, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human VEGFD.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes vascular endothelial growth factor D as a secreted protein. Tissue expression noted by UniProt (human): Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yin Bo-hao et al. (2020) Effects of hypoxia environment on osteonecrosis of the femoral head in Sprague-Dawley rats. Journal of Bone and Mineral Metabolism. 10.1007/s00774-020-01114-0
