Anti-VEGI Unconjugated

Overview

Anti-VEGI Unconjugated is a Mouse monoclonal targeting VEGI, supplied as a Unconjugated format for ICC / WB workflows. It supports measurement of Human target expression in common experimental systems.

Key elements and design rationale

• Clone: 1-8F-7G — consistent clone identity can support panel reproducibility and cross-study comparisons.
• Isotype: IgG1, k — informs selection of matched controls and secondary reagents when relevant.
• Conjugate: Unconjugated — enables direct detection in fluorescence-based assays.
• Host species: Mouse — useful for panel design and control strategy planning.
• Reactivity: Human — interpret staining in the context of species-specific sequence and expression differences.

Key specifications such as clone identity, isotype, and fluorophore conjugation help researchers align panel design, control selection, and instrument configuration with the biological question and sample type.

Biological background

Vascular endothelial cell growth inhibitor (VEGI), a member of the tumor necrosis factor (TNF) family, is an endothelial cell-specific inhibitor of angiogenesis. Overexpression of a secretable VEGI fusion protein by cancer cells resulted in abrogation of xenograft tumor progression. 4-3H8B is a mouse monoclonal IgG1 raised against a recombinant VEGI isoform which shares a C-terminal 151-residue segment with previously reported VEGI with 174 residues.

Research relevance and current trends

• High-parameter immunophenotyping: combining VEGI with complementary lineage and activation markers to resolve complex cell states.
• Panel standardization and data comparability: increasing emphasis on consistent reagents, compensation-aware fluorophore choices, and shared gating strategies.
• Integration with single-cell multi-omics: pairing surface marker profiling with transcriptomic or proteomic readouts to connect phenotype to function.

Common research applications

• Immunofluorescence: visualize cellular distribution of VEGI and assess co-localization with other markers.
• Western blot: evaluate VEGI expression changes and consider isoforms or PTMs when interpreting band patterns.

Changes in measured signal are typically interpreted in the context of cell subset frequency, activation/differentiation state, and sample processing effects rather than as a standalone readout.

Notes for experimental interpretation

• Fluorophore selection: consider brightness, spectral overlap, and instrument configuration; compensation and spillover can affect apparent population boundaries.
• Biology-driven confounders: activation state, differentiation, and isoform/PTM variation can shift epitope accessibility and apparent expression.
• Control concepts: include matched isotype and fluorescence-minus-one (FMO) controls where appropriate, and interpret results alongside biological positive/negative reference samples.

For antibody-based assays, monoclonal versus polyclonal format can influence epitope recognition breadth and signal consistency. Conjugated antibodies support direct detection and can simplify multicolor panel design when paired with appropriate controls and instrument settings.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.