| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vesicular glutamate transporter 1; VGluT1; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7; SLC17A7; BNPI |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VGluT1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18S50 is a rabbit monoclonal antibody that recognizes vesicular glutamate transporter 1 (gene SLC17A7) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 61.6 kDa, and the supplier reports an observed band at about 53 kDa.
Vesicular glutamate transporter 1 (VGLUT1, SLC17A7) fills synaptic vesicles with glutamate, driven by the proton electrochemical gradient across the vesicle membrane. It predominates in excitatory neurons of the cerebral cortex, hippocampus and cerebellar cortex, and it is a standard marker of glutamatergic synapses.
| Target | Vesicular glutamate transporter 1 (gene SLC17A7; UniProt Q9P2U7, human) |
|---|---|
| Clone | 18S50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.6 kDa |
| Observed MW | 53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human VGluT1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places vesicular glutamate transporter 1 in the cell membrane and synapse. Tissue expression noted by UniProt (human): Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
