| Field | Specification |
|---|---|
| Target | |
| Alternative names | Villin-1; VIL1; VIL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Villin Ca (2+) -regulated actin-binding protein. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFOA-22 is a rabbit monoclonal antibody that recognizes villin-1 (gene VIL1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 92.7 kDa, and the supplier reports an observed band at about 93 kDa.
Villin-1 is an actin-binding protein of the brush border microvilli in intestinal and kidney proximal tubule epithelium. Depending on calcium levels, it bundles, caps or severs actin filaments, shaping microvillus structure. Villin is widely used as a marker of intestinal epithelial differentiation.
| Target | Villin-1 (gene VIL1; UniProt P09327, human) |
|---|---|
| Clone | AFOA-22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.7 kDa |
| Observed MW | 93 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human villin Ca (2+) -regulated actin-binding protein.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places villin-1 in the cytoplasm and cell projection. Tissue expression noted by UniProt (human): Specifically expressed in epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
