| Field | Specification |
|---|---|
| Target | |
| Gene symbol | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide of human Vimentin |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone R02-8C4), raised in Rabbit, recognizes VIM. It is validated for Western Blot, IHC-F, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | Western Blot, IHC-F, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB 1 | 500-1:1000 |
| IHC 1 | 50-1:100 |
| IF 1 | 50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthetic peptide of human Vimentin
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Clone | R02-8C4 |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Hamster |
| Immunogen | A synthetic peptide of human Vimentin |
| Immunogen Region | 448-465 aa |
| Purification | Affinity Purified |
| Concentration | 0.3 mg/mL |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| UniProt # | P08670 |
| Gene ID | 7431 |
| Storage | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat, Hamster. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for VIM research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
