Anti-Vinculin/VCL Antibody Picoband®

SKU:BHA21007049
Research Validated
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-VCL antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot and immunohistochemistry. Reactivity: Mouse (species coverage varies by application). Recognizes vinculin. Observed band in Western blot: about 34 kDa.
Target VCL
Host Rabbit
Reactivity Mouse
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
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Catalog no. Size Conjugation
PA1781 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated; 100 ug/vial — Carrier Free
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
      Form: Lyophilized
      Applications: IHC,WB
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
      Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial — Biotin; 100 ug/vial — Cy3; 100 ug/vial — Fluoro488; 100 ug/vial — Fluoro550; 100 ug/vial — Fluoro594; 100 ug/vial — FITC; 100 ug/vial — APC; 100 ug/vial — PE; 100 ug/vial — Fluoro647
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      Form: Liquid
      Applications: Flow Cytometry | WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial — HRP
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
      Form: Liquid
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target VCL
Alternative names Vinculin;Metavinculin;MV;VCL;
Gene ID 7414
UniProt # P18206
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Mouse
Applications
  • Western Blot
  • Immunohistochemistry
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin, identical to the related rat and mouse sequences.
Molecular weight 123.8 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions. .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) PA1781
Main SKU BHA21007049
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody detects vinculin (gene VCL) in mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 123.8 kDa, and the supplier reports an observed band at about 34 kDa.

Vinculin is an actin-binding protein found at focal adhesions and adherens junctions. It links integrin- and cadherin-based adhesions to the actin cytoskeleton and helps these junctions respond to mechanical force. Vinculin is a standard marker of focal adhesions in studies of cell adhesion, migration and mechanobiology.

Target Vinculin (gene VCL; UniProt P18206, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Mouse
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers
Calculated MW 123.8 kDa
Observed MW 34 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 0.5–1 µg/mL (Human, Rat, Mouse)
Western blot 0.1–0.5 µg/mL (Human, Mouse, Rat)

Samples with a confirmed band (WB): rat heart tissue, rat brain tissue, rat liver tissue, U87 cells, SMMC cells, HEPA cells, HeLa cells, HT1080 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Samples with confirmed staining (IHC): human mammary cancer tissue, rat intestine tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Immunogen

Synthetic peptide from the N-terminal region of human vinculin. The peptide sequence is conserved in rat and mouse.

Reactivity Notes

The supplier lists reactivity with mouse. UniProt places vinculin in the cell membrane, cell junction and cytoplasm. Tissue expression noted by UniProt (human): Metavinculin is muscle-specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide and thimerosal (a mercury compound) as preservatives. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Human, Mouse, Rat); IHC (paraffin sections) 0.5–1 µg/mL (Human, Rat, Mouse). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse. Other species are not listed by the supplier.
Q.Has this antibody been tested on monkey samples?
A.Reactivity with monkey samples has not been tested by the supplier. Aligning the immunogen sequence with the monkey ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 123.8 kDa; observed band at about 34 kDa. The observed band is much smaller than the calculated size, which can indicate a processed form or a shorter isoform; check the supplier images.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide from the N-terminal region of human vinculin. The peptide sequence is conserved in rat and mouse.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Miusi Shi et al. (2022) Oxygen Ion Implantation Improving Cell Adhesion on Titanium Surfaces through Increased Attraction of Fibronectin PHSRN Domain. Advanced Healthcare Materials. 10.1002/adhm.202101983

Wenting Mo et al. (2021) Customized protein modification improves human gingival fibroblasts adhesion on SiO2. Applied Materials Today. 10.1016/j.apmt.2021.101232

Haibin Wu et al. (2017) Ceria nanocrystals decorated mesoporous silica nanoparticle based ROS-scavenging tissue adhesive for highly efficient regenerative wound healing. Biomaterials. 10.1016/j.biomaterials.2017.10.018

Liu Shuhui et al. (2025) Cellular hnRNP AB inhibits avian influenza virus RNA synthesis via blocking UAP56-mediated nuclear export of PB2 mRNA. Veterinary Research. 10.1186/s13567-025-01660-3

Hongliu Ye et al. (2021) Cellular hnRNPAB interacts with avian influenza viral protein PB2 and inhibits virus replication potentially by restricting PB2 mRNA nuclear export and PB2 protein level. Virus Research. 10.1016/j.virusres.2021.198573

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