| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vitamin D3 receptor;VDR;1,25-dihydroxyvitamin D3 receptor;Nuclear receptor subfamily 1 group I member 1;VDR;NR1I1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Vitamin D Receptor |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAGE-22 is a rabbit monoclonal antibody that recognizes vitamin D3 receptor (gene VDR) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 48 kDa.
The vitamin D receptor (VDR) is a nuclear receptor activated by calcitriol, the active form of vitamin D. Bound to its ligand, it pairs with the retinoid X receptor and binds vitamin D response elements to control target genes. VDR is central to calcium and phosphate balance and bone mineralization, and is also studied in immunity and cell differentiation.
| Target | Vitamin D3 receptor (gene VDR; UniProt P11473, human) |
|---|---|
| Clone | AAGE-22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.3 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human vitamin D Receptor.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places vitamin D3 receptor in the nucleus and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
