| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vitronectin;VN;S-protein;Serum-spreading factor;V75;Vitronectin V65 subunit;Vitronectin V10 subunit;Somatomedin-B;VTN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Vitronectin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DCC-22 is a rabbit monoclonal antibody that recognizes vitronectin (gene VTN) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 54.3 kDa, and the supplier reports an observed band at about 75 kDa.
Vitronectin is a glycoprotein abundant in plasma and the extracellular matrix. It binds integrins such as αvβ3 to support cell adhesion and spreading, stabilizes the protease inhibitor PAI-1, and limits formation of the membrane attack complex of complement. Vitronectin is studied in cell adhesion, fibrinolysis and tissue repair.
| Target | Vitronectin (gene VTN; UniProt P04004, human) |
|---|---|
| Clone | DCC-22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.3 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human Serum membrane.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human vitronectin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes vitronectin as a secreted protein. Tissue expression noted by UniProt (human): Expressed in the retina pigment epithelium (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
