| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vacuolar protein sorting-associated protein 11 homolog; hVPS11; RING finger protein 108; VPS11; RNF108 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VPS11 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22V84 is a rabbit monoclonal antibody that recognizes vacuolar protein sorting-associated protein 11 homolog (gene VPS11) in human and mouse samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 107.8 kDa, and the supplier reports an observed band at about 108 kDa.
VPS11 is a core subunit shared by the HOPS and CORVET tethering complexes of the endolysosomal system. These complexes bind Rab GTPases and SNAREs to dock and fuse endosomes, lysosomes and autophagosomes. VPS11 is studied in lysosomal trafficking and autophagy.
| Target | Vacuolar protein sorting-associated protein 11 homolog (gene VPS11; UniProt Q9H270, human) |
|---|---|
| Clone | 22V84 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 107.8 kDa |
| Observed MW | 108 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:80 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human VPS11.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places vacuolar protein sorting-associated protein 11 homolog in the endosome, late endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
