| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vacuolar protein sorting-associated protein 28 homolog; H-Vps28; ESCRT-I complex subunit VPS28; VPS28 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VPS28 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25V63 is a rabbit monoclonal antibody that recognizes vacuolar protein sorting-associated protein 28 homolog (gene VPS28) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 25.4 kDa, and the supplier reports an observed band at about 25 kDa.
VPS28 is a subunit of ESCRT-I, a complex that recognizes ubiquitinated membrane proteins on endosomes and helps sort them into multivesicular bodies for lysosomal degradation. ESCRT-I also takes part in virus budding and cytokinesis.
| Target | Vacuolar protein sorting-associated protein 28 homolog (gene VPS28; UniProt Q9UK41, human) |
|---|---|
| Clone | 25V63 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 25.4 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human VPS28.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places vacuolar protein sorting-associated protein 28 homolog in the cell membrane and late endosome membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
