| Field | Specification |
|---|---|
| Target | |
| Alternative names | von Willebrand factor;vWF;von Willebrand antigen 2;von Willebrand antigen II;VWF;F8VWF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human VWF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IHF-22 is a rabbit monoclonal antibody that recognizes von Willebrand factor (gene VWF) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 309.3 kDa, and the supplier reports an observed band at about 309 kDa.
Von Willebrand factor (VWF) is a large multimeric plasma glycoprotein made by endothelial cells and megakaryocytes. At sites of vessel injury it bridges exposed collagen to the platelet receptor GPIb, supporting platelet adhesion, and it carries and protects coagulation factor VIII. Its deficiency causes von Willebrand disease, the most common inherited bleeding disorder.
| Target | Von Willebrand factor (gene VWF; UniProt P04275, human) |
|---|---|
| Clone | IHF-22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 309.3 kDa |
| Observed MW | 309 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human serum.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human VWF.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes von Willebrand factor as a secreted protein. Tissue expression noted by UniProt (human): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
