| Field | Specification |
|---|---|
| Target | |
| Alternative names | Wings apart-like protein homolog; Friend of EBNA2 protein; WAPL cohesin release factor; WAPL; FOE; KIAA0261; WAPAL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human WAPL |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22W59 is a rabbit monoclonal antibody that recognizes wings apart-like protein homolog (gene WAPL) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 132.9 kDa, and the supplier reports an observed band at about 160 kDa.
WAPL is a regulator of cohesin, the ring complex that holds sister chromatids together. It opens the cohesin ring to release it from chromatin, which limits chromatin loop size in interphase and removes most cohesin from chromosome arms in early mitosis. WAPL is studied in chromosome segregation and 3D genome organization.
| Target | Wings apart-like protein homolog (gene WAPL; UniProt Q7Z5K2, human) |
|---|---|
| Clone | 22W59 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 132.9 kDa |
| Observed MW | 160 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human WAPL.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Isoform 1 is highly expressed in uterine cervix tumor. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
