| Field | Specification |
|---|---|
| Target | |
| Alternative names | Actin nucleation-promoting factor WAS; Wiskott-Aldrich syndrome protein; WASp; WAS; IMD2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human WASP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23W45 is a rabbit monoclonal antibody that recognizes actin nucleation-promoting factor WAS in human, mouse and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 52.9 kDa, and the supplier reports an observed band at about 60 kDa.
WASP is an actin nucleation-promoting factor expressed in blood cells. Activated by CDC42, it stimulates the Arp2/3 complex to build branched actin networks needed for immune synapse formation, migration and phagocytosis. Mutations cause Wiskott–Aldrich syndrome, an immunodeficiency with small platelets and eczema.
| Target | Actin nucleation-promoting factor WAS (gene WAS; UniProt P42768, human) |
|---|---|
| Clone | 23W45 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 52.9 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:40 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Jurkat cells, human HEL cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human WASP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places actin nucleation-promoting factor WAS in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed predominantly in the thymus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
