| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Vesicular acetylcholine transporter;VAChT;Solute carrier family 18 member 3;SLC18A3;VACHT; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human WDR77 recombinant protein (Position: A13-A295). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-WDR77 Antibody Picoband® is an antibody for WDR77 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: WDR77 (Vesicular acetylcholine transporter); UniProt: Q9BQA1
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 42 kDa, calculated 56903 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-WDR77 Antibody Picoband® catalog # A04894-1.
Biological background
Biological context: Involved in acetylcholine transport into synaptic vesicles. .
Expression and localization notes: cellular localization: Membrane ; Multi-pass membrane protein ., tissue context: Peripheral and central cholinergic nervous systems. ..
Common research applications
- Western blotting (WB): Compare WDR77 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of WDR77 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify WDR77-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane ; Multi-pass membrane protein .
- Tissue details: Peripheral and central cholinergic nervous systems. .
- Research category: Neuroscience,Neurotransmitter
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
