| Field | Specification |
|---|---|
| Target | |
| Alternative names | WAS/WASL-interacting protein family member 3; Corticosteroids and regional expression protein 16 homolog; WIPF3; CR16 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human WIPF3, which shares 95.5% and 90.9% amino acid (aa) sequence identity with mouse and rat WIPF3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets WAS/WASL-interacting protein family member 3 (gene WIPF3) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 49.5 kDa, and the supplier reports an observed band at about 49 kDa.
WIPF3 (also called CR16) is a member of the WASP-interacting protein family, a group of proline-rich proteins that bind WASP-family actin regulators. It is thought to take part in cytoskeletal organization and has been suggested to have a role in spermatogenesis, but its function is not yet well characterized.
| Target | WAS/WASL-interacting protein family member 3 (gene WIPF3; UniProt A6NGB9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 49.5 kDa |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human K562 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Synthetic peptide from the C-terminal region of human WIPF3. Sequence identity with the mouse and rat orthologs: 95.5% and 90.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places WAS/WASL-interacting protein family member 3 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
