Anti-Wnt2b Antibody Picoband®

Overview

Anti-Wnt2b Antibody Picoband® is an antibody for WNT2B detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: WNT2B (protein O-mannosyltransferase 2); UniProt: Q93097
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 60 kDa, calculated 35883 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Wnt2b Antibody Picoband® catalog # A04879-1.

Biological background

Biological context: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.

Expression and localization notes: cellular localization: Endoplasmic reticulum membrane. Multi-pass membrane protein, tissue context: Highly expressed in testis; detected at low levels in most tissues..

Common research applications

• Western blotting (WB): Compare WNT2B levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of WNT2B in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify WNT2B-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Endoplasmic reticulum membrane. Multi-pass membrane protein
• Tissue details: Highly expressed in testis; detected at low levels in most tissues.
• Research category: Organelles,Subcellular Markers,Tags & Cell Markers

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.