| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein Wnt-5a;WNT5A; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Wnt5a recombinant protein (Position: D184-D303). Human Wnt5a shares 100% amino acid (aa) sequence identity with both mouse and rat Wnt5a. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein Wnt-5a (gene WNT5A) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 45 kDa.
Wnt-5a is a secreted Wnt ligand best known for non-canonical signaling, acting through receptors such as ROR2 and Frizzled to regulate cell polarity and migration. Depending on receptor context it can either activate or oppose β-catenin signaling, and it is required for outgrowth of the body axis, limbs and genitalia, so it is studied in development, inflammation and cancer.
| Target | Protein Wnt-5a (gene WNT5A; UniProt P41221, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 42.3 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Wnt5a fragment (Asp184–Asp303), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes protein Wnt-5a as a secreted protein. Tissue expression noted by UniProt (human): Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Yu-Biao et al. (2024) Wnt5a-mediated autophagy contributes to the epithelial-mesenchymal transition of human bronchial epithelial cells during asthma. Molecular Medicine. 10.1186/s10020-024-00862-3
Zhong Gang et al. (2018) Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway. Cellular Physiology and Biochemistry. 10.1159/000495926
Wei Jia et al. (2018) Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells. International Journal of Oncology. 10.3892/ijo.2018.4330
Liu Jingyi et al. (2016) Aberrant activation of Wnt pathways in arteries associates with vascular calcification in chronic kidney disease. International Urology and Nephrology. 10.1007/s11255-016-1291-2
