| Field | Specification |
|---|---|
| Target | |
| Alternative names | Xanthine dehydrogenase/oxidase; XDH; XDHA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Xanthine Oxidase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19X78 is a rabbit monoclonal antibody that recognizes xanthine dehydrogenase/oxidase (gene XDH) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 146.4 kDa, and the supplier reports an observed band at about 146 kDa.
Xanthine dehydrogenase/oxidase (XDH) is a molybdenum-containing enzyme that catalyzes the last two steps of purine breakdown, oxidizing hypoxanthine to xanthine and xanthine to uric acid. It can exist as a dehydrogenase or be converted to an oxidase form that generates reactive oxygen species, and it is the target of urate-lowering drugs such as allopurinol, making it relevant to gout and oxidative stress research.
| Target | Xanthine dehydrogenase/oxidase (gene XDH; UniProt P47989, human) |
|---|---|
| Clone | 19X78 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 146.4 kDa |
| Observed MW | 146 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse small intestine tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human xanthine Oxidase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes xanthine dehydrogenase/oxidase as secreted and also located in the cytoplasm and peroxisome. Tissue expression noted by UniProt (human): Detected in milk (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shulan Qi et al. (2024) Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation. Bioelectrochemistry. 10.1016/j.bioelechem.2024.108832
Wuyang Huang et al. (2016) Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells. Oxidative Medicine and Cellular Longevity. 10.1155/2016/1591803
