| Field | Specification |
|---|---|
| Target | |
| Alternative names | Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor; NHEJ1; XLF |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human XLF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22N07 is a rabbit monoclonal antibody that recognizes non-homologous end-joining factor 1 (gene NHEJ1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 33.3 kDa, and the supplier reports an observed band at about 39 kDa.
XLF (also called Cernunnos, NHEJ1) is a core factor of non-homologous end joining, the main pathway for repairing DNA double-strand breaks in mammalian cells. It works with XRCC4 and DNA ligase IV to align and join broken ends and is needed for V(D)J recombination during lymphocyte development.
| Target | Non-homologous end-joining factor 1 (gene NHEJ1; UniProt Q9H9Q4, human) |
|---|---|
| Clone | 22N07 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.3 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human XLF.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places non-homologous end-joining factor 1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
