| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA repair protein XRCC1;X-ray repair cross-complementing protein 1;XRCC1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human XRCC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABOH-24 is a rabbit monoclonal antibody that recognizes DNA repair protein XRCC1 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 69.5 kDa, and the supplier reports an observed band at about 90 kDa.
XRCC1 is a scaffold protein without enzymatic activity that organizes repair of DNA single-strand breaks and base excision repair. It binds poly(ADP-ribose) on activated PARP1 and brings together partners such as DNA ligase III and DNA polymerase β, while limiting excessive PARP1 activity, and it is studied in genome stability and responses to DNA-damaging agents.
| Target | DNA repair protein XRCC1 (gene XRCC1; UniProt P18887, human) |
|---|---|
| Clone | ABOH-24 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.5 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human XRCC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA repair protein XRCC1 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haiyan Zhang et al. (2024) PARP1 inhibitor niraparib exerts synergistic antimyeloma effect with bortezomib through inducing DNA damage and inhibiting DNA repair. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2024.12.052
